![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2557019:Design
Designed by: Ge JT Group: iGEM18_NAU-CHINA (2018-10-10)
miniCMV promoter-Bxb1-RDF
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1612
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1612
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 820
Illegal BglII site found at 1618
Illegal BamHI site found at 46
Illegal BamHI site found at 261
Illegal BamHI site found at 577
Illegal BamHI site found at 1285
Illegal BamHI site found at 1576 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1612
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1612
Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 2095
Illegal NgoMIV site found at 2278
Illegal AgeI site found at 52 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 282
Design Notes
In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.
Source
The miniCMV promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the sequence of Bxb1-RDF from the literature and synthesized this sequence.